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. Author manuscript; available in PMC: 2010 Apr 9.
Published in final edited form as: Blood. 2003 Aug 7;102(12):3963–3969. doi: 10.1182/blood-2003-05-1490

Figure 5. T lymphocytes immortalized by HTLVRex− contain the expected Rex− mutation and express tax/rex mRNA.

Figure 5

(A) HTLV-1 genome fragment containing the Rex start codon was amplified by PCR from high molecular weight DNA of wtHTLV-1 and HTLVRex− immortalized cells. PCR-amplified product was incubated in the presence or absence of SphI, separated on 2% agarose gel, and visualized by ethidium bromide staining. (B) Detection of tax/rex mRNA in the wtHTLV-1 and HTLVRex− immortalized PBMCs. Total RNA was prepared from fresh PBMCs, wtHTLV-1, and HTLVRex− immortalized PBMCs. Approximately 0.3 µg RNA was subjected to a coupled 40-cycle RT-PCR in the presence (+) or absence (−) of reverse transcriptase (RT). Following RT-PCR, 25-cycle nested PCR was performed. PCR product was separated on 2% agarose gel and visualized by ethidium bromide staining.