Table 2. Comparison of strategies for insect-cell expression of SeMet-labelled protein.
SeMet (mg l−1) | Yield (% that of unlabelled expression) | Incorporation of SeMet (%) |
---|---|---|
50–100 | ∼50 | ∼50 |
200 | ∼20 | >70 |
SeMet (mg l−1) | Yield (% that of unlabelled expression) | Incorporation of SeMet (%) |
---|---|---|
50–100 | ∼50 | ∼50 |
200 | ∼20 | >70 |