Table 3. Derivatization conditions and phasing statistics of lysozyme derivatives (adapted from Sun et al., 2002 ▶).
| KAuCl4 | K2PtCl6 | |||||||
|---|---|---|---|---|---|---|---|---|
| Derivatization† | 10 mM, 10min | 10 mM, 24h | 1 mM, 48h | 10 mM, 10min | 12 mM, 10min | 1 mM, 10min | 1 mM, 22h | 10 mM, 48h |
| R iso | 0.201 | 0.462 | 0.349 | 0.176 | 0.208 | 0.111 | 0.087 | 0.213 |
| Heavy-atom peak height‡ | ||||||||
| Site 1 () | 21.6 | 4.0 | 15.7 | 19.3 | 18.2 | 5.0 | 15.0 | 6.2 |
| Site 2 () | 12.8 | 4.0 | 9.3 | 16.3 | 16.5 | 5.0 | 10.5 | 11.0 |
| Site 3 () | 9.7 | 4.0 | ||||||
†
Heavy-atom soaking concentration, soaking time.
‡
The heavy-atom sites are shown as peak heights in standard deviations from the difference Fourier (F PH F P) map. For the KAuCl4 derivative the coordinates of sites 1, 2 and 3 are (11.36, 11.72, 19.21), (8.49, 10.2, 14.25) and (3.30, 7.94, 9.84) , respectively. For the K2PtCl6 derivative the coordinates of site 1 and 2 are (10.957, 10.957, 9.23) and (6.143, 3.859, 29.992) , respectively.