Table 4. Derivatization conditions and phasing statistics of FcRIII derivatives (adapted from Sun et al., 2002 ▶).
| TMLA | HgCl2 | ||||
|---|---|---|---|---|---|
| Derivatization† | 5 mM, 10min | 10 mM, 10min | 10 mM, 24h | Saturated, 10min | Saturated, 2h |
| R iso | 0.093 | 0.09 | 0.168 | 0.119 | 0.273 |
| Heavy-atom peak height‡ | |||||
| Site 1 () | 6.7 | 17.8 | 5.0 | 7.6 | 24.4 |
| Site 2 () | 6.0 | 12.8 | 5.0 | 5.2 | 16.4 |
†
Heavy-atom soaking concentration, soaking time.
‡
The heavy-atom sites are shown as peak heights in standard deviations from the difference Fourier (F PH F P) map. The coordinates of sites 1 and 2 of the TMLA derivatives are (111.99, 12.54, 13.41) and (88.49, 21.42, 23.78) , respectively. The coordinates of sites 1 and 2 of the HgCl2 derivatives are (80.27, 1.80, 27.71) and (104.52, 7.78, 29.52) , respectively