Table 5. Rational heavy-atom screening of lysozyme (adapted from Agniswamy et al., 2008 ▶).
The extent of heavy-atom reactivity was evaluated based on the peak heights of observed derivatives from mass-spectrometric experiments and was assigned on a four-level scale as either , +, ++ or +++, which equate to no significant derivative adduct formation and derivative adducts with peak heights less than 25%, between 25 and 50% and above 50% of the native peak intensity, respectively.
| Compound | Peptide reactivity | Lysozyme reactivity |
|---|---|---|
| MHTS | ND | Blake et al. (1962 ▶) |
| K2PdCl4 | ND | Blake et al. (1962 ▶) |
| K2HgBr4 | ND | Blake (1968 ▶) |
| K2HgI4 | Blake et al. (1962 ▶) | |
| PCMB | Blake (1968 ▶) | |
| PCMBS | + | Blake (1968 ▶) |
| K2PtCl6 | + | Blake (1968 ▶) |
| K2AuCl4 | ++ | Blake (1968 ▶) |
| K2PtBr4 | +++ | +; Blake (1968 ▶) |
| K2PtCl4 | +++ | +++; Blake (1968 ▶) |
| K2PtBr6 | +++ | +++ |
| Methylmercury(II) acetate | +++ | +++ |
| Ethylmercury phosphate | +++ | +++ |
| Mercury(II) acetate | +++ | +++ |
| TELA | +++ | + |
| Lead nitrate | +++ | +++ |
| Lead acetate | +++ | +++ |
| Diaminoplatinum dinitrate | +++ | + |
| Gold(II) chloride | +++ | + |
| Thiomersal | +++ | |
| Mersalyl | +++ | |
| Mercury(II) bromide | +++ | |
| Methylmercury(II) chloride | +++ | |
| Mercury(II) iodide | ||
| Methylmercury(II) bromide | ||
| K2Pt(CN)4 | +++ | |
| K2PtI6 | ||
| Gold sodium thiosulfate | ||
| Hexaphenyllead |