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. 2010 Mar 31;66(Pt 4):426–430. doi: 10.1107/S1744309110005002

Figure 2.

Figure 2

(a) Crystals of cVLCAD grown under condition No. 92 of the Index kit (100 mM magnesium formate, 15% PEG 3350). 5 mg ml−1 protein buffered in 20 mM Tris–HCl pH 8.0, 150 mM sodium chloride was used. (b) Crystals of cVLCAD grown using 10 mg ml−1 protein solution (buffered in 20 mM Tris–HCl pH 8.0, 150 mM sodium chloride), 100 mM Tris–HCl pH 8.0, 100 mM magnesium formate, 15% PEG 3350. (c) Crystals of cVLCAD grown using 12 mg ml−1 protein solution (buffered in 20 mM Tris–HCl pH 8.0, 150 mM sodium chloride), 100 mM Tris–HCl pH 8.0, 200 mM magnesium formate, 13% PEG 3350. cVLCAD has a yellow colour.