Table 1. Data-collection statistics.

Values in parentheses are for the highest resolution shell.

	TP_nat	TP_glc	TP_OG	TP_tre	TP_mut
X-ray source	SLS X06SA	BESSY ID14.1	SLS X06SA	BESSY ID14.2	SOLEIL PROXIMA1
Temperature (K)	100	100	100	100	100
Dehydration	No	No	No	No	Method 1
Cryoprotectant	PEG 4000	PEG 4000	MPD	MPD	MPD
Ligand	None	n-Octyl-β-D-glucose	Glucose	Trehalose	Trehalose + Pi
Ligand concentration	—	1%(w/v)	2%(w/v)	0.4%(w/v)	0.75%(w/v) trehalose, 19 mM phosphate
Method for ligand complexes	—	Cocrystallization	Transfer soak (2 h)	Addition soak (30 min)	Addition soak (56 h)
Wavelength (Å)	0.9998	0.9184	0.9999	0.9184	0.9184
Data range (°)	113	140	100	100	140
Frame oscillation (°)	0.125	0.2	0.2	0.2	0.2
Data processing	XDS/XSCALE	XDS/XSCALE	XDS/XSCALE	XDS/XSCALE	XDS/XSCALE
Space group	P212121	P212121	P212121	P212121	P212121
Resolution (Å)	50–2.8	40–4.6	30–4.2	30–3.4	30–4.1
Unit-cell parameters
a (Å)	97.84	102.4	103.8	98.39	101.2
b (Å)	104.28	105.2	105.4	103.92	107.4
c (Å)	415.71	423.1	424.0	420.6	422.0
Molecules in asymmetric unit	4	4	4	4	4
Unique reflections	100648	26162	34229	59496	36988
Redundancy	4.00	5.62	4.09	3.29	5.55
Mosaicity (°)	0.24	0.19	0.15	0.24	0.19
Completeness (%)	95.1 (96.1)	98.3 (96.7)	98.0 (96.2)	98.4 (99.7)	99.3 (99.3)
Rmeas† (%)	11.0 (76.5)	16.8 (93.2)	9.8 (50.5)	16.2 (73.6)	7.4 (77.3)
〈I/σ(I)〉	10.2 (2.4)	9.08 (2.12)	12.46 (3.05)	8.55 (2.44)	14.02 (2.55)
RFZ‡	6.1, 3.9, 3.7, 3.8
TFZ‡	4.4, 12.4, 19.3, 14.2
LLG‡	58, 1884, 381, 821

^† R _meas is the multiplicity-weighted R _merge, i.e. , where I_i(hkl) is the intensity of the ith measurement of reflection hkl and 〈I(hkl)〉 is the average value over multiple measurements (Diederichs & Karplus, 1997 ▶).

^‡For monomers 1, 2, 3 and 4, respectively.