Figure 5.

Creatine kinase (CK)-binding specificities of human ankyrin repeat and suppressor of cytokine signalling (SOCS) box protein 9 (ASB9). (a) Expression of CKs in human normal hepatocytes human hepatoma cell lines and normal human hepatocytes. Whole lysates were subjected to immunoblotting (IB) with anti-CKB, anti-ubiquitous mitochondrial CK (uMtCK), and anti-CKM, and anti-sarcomeric MtCK (sMtCK) antibodies. The β-actin expression level was used as a control. (b) Association of ASB9 with CKB and uMtCK. The human embryonic kidney 293 calls (HEK293) cells were stably transfected with empty, Myc-tagged ASB9 and ASB9ΔSOCS expression vectors. Whole lysates or immunoprecipitates obtained with the anti-Myc antibody were subjected to immunoblotting (IB) analysis with anti-Myc, anti-CKB, anti-uMtCK, and anti-elongin B antibodies.