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. 2010 Mar 19;8:23. doi: 10.1186/1741-7007-8-23

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Copyright ©2010 Kwon et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Interaction of human ankyrin repeat and suppressor of cytokine signalling (SOCS) box protein 9 (ASB9) with substrate binding site of ubiquitous mitochondrial creatine kinase (uMtCK). (a) Schematic diagram of the uMtCK and an uMtCK substrate binding site (BS) deletion mutant. (b) A substrate binding site of uMtCK is required for ASB9 interaction. Haemagglutinin (HA)-tagged uMtCK or uMtCKΔBS were transiently cotransfected with Myc-tagged ASB9 or ASB9ΔSOCS into human embryonic kidney 293 (HEK293) cells, and immunocomplexing assays were performed with an anti-Myc antibody. Cell lysates and immunoprecipitates were analysed by immunoblotting with anti-Myc or anti-HA antibodies.