Figure 1. Analysis of receptor for advanced glycation end-products (RAGE) expression and RAGE ligands subjected to hypoxia followed by reoxygenation.

WT cardiomyocytes were collected and lysates obtained at the end of normoxia (N), 30 min of hypoxia (H), and hypoxia (30 min) followed by 1 hr reoxygenation (HR), were subjected to Western blot analysis (A–B) and ELISA (C) for the detection of RAGE and its ligands. Cell lysate was probed with (A) anti-RAGE antibody; (B) anti-CML antibody. After being probed with the target antibodies, blots were stripped and reprobed with anti-β-actin IgG. Relative density units are reported. n = 3. (C) 100 µg/well protein was coated and analyzed by ELISA for detection of heterogeneous AGE epitopes. Each sample was measured in two parallel wells and experiment was repeated three times.