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. 2010 Apr 9;5(4):e10092. doi: 10.1371/journal.pone.0010092

Figure 4. Inhibition of JNK alleviates the injury due to H/R in cardiomyocytes.

Figure 4

WT cardiomyocytes were subjected to hypoxia/reoxygenation after 1 hr incubation with JNK inhibitor SP600125 (10 µM) or the vehicle control DMSO. (A) Cell supernatant was collected for LDH level measurement. n = 3. (B) Cleaved caspase 3 levels and (C) Cytochrome c were detected after hypoxia/reoxygenation by Western blot analysis. Data are representative of three independent experiments. (D) Cardiomyocytes isolated from WT and RKO and sRAGE-treated mice were incubated with JNK inhibitor SP600125 (10 µM) or its vehicle control DMSO for 1 hr, followed by hypoxia 30 mins/reoxygenation 1 hr treatment. Cell supernatant was collected for LDH release measurement. (E) The hearts of RKO mice were pre-perfused with JNK-specific inhibitor SP600125 or its negative control for 30 mins prior to the cardiomyocyte isolation process. Cell supernatant was collected for LDH level measurement. Pre-perfusion with the JNK inhibitor did not abrogate the protective effects of RAGE deletion in H/R. n = 3. SP: SP600125.