Figure 5. Deletion of RAGE promotes cardiomyocyte survival in H/R by enhancing phosphorylation of GSK3β.

(A) Cells were collected and lysates obtained at the end of normoxia (N), 30 mins of hypoxia (H), and hypoxia (30 min) followed by 1 hr reoxygenation (HR). Lysates from WT, RKO and sRAGE-treated cardiomyocytes were subjected to Western blot analysis for the detection of phospho-S9-GSK3β and total-GSK3β. n = 3. (B) Cardiomyocytes were incubated with or without GSK3 inhibitors LiCl (12.5 mM) and SB216763 (3 µM) for 1 hr, followed by 30 min hypoxia/1 hr reoxygenation (HR), cell supernatant was collected for LDH release measurement. n = 3.