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. 2010 Apr 9;5(4):e10092. doi: 10.1371/journal.pone.0010092

Figure 6. Akt/PKB contributes to the phosphorylation of GSK.

Figure 6

(A) Cells were collected and lysates obtained at the end of normoxia (N), 30 min of hypoxia (H), hypoxia (30 min) followed by 1 hr reoxygenation (HR). Lysates from WT, RKO and sRAGE-treated cardiomyocytes subjected to Western blot analysis for the detection of phospho-Akt and total-Akt. n = 3. (B) Cardiomyocytes were incubated with LY294002 (10 µM) or its vehicle control DMSO for 1 hr, followed by HR. Cell supernatant was collected for LDH release measurement. n = 3. (C–D) Cardiomyocytes were incubated with LY294002 (10 µM) or its vehicle control DMSO for 1 hr, followed by HR. Cell lysate was collected for Western blot studies for detection of phospho/total Akt (C) and GSK3β (D). n = 3. LY: LY294002.