Figure 2. Gel filtration chromatograms documenting the final purification step of Nef and myristoylated Nef (myr. Nef).

Protein samples were loaded onto a preparative HiLoad 16/60 Superdex 75 column, eluted with a flow rate of 1 ml/min and the absorbance at 280 nm was recorded. The elution profiles with maxima of the main peaks at around 61 ml indicate a monomeric state of Nef. Minor fractions eluting at around 44 and 51 ml reflect traces of aggregated protein and GST-PreScission protease, respectively.