Figure 3. SDS-PAGE of 2 ml fractions collected during the final gel filtration of myristoylated Nef.

Aliquots of the SEC fractions of myristoylated Nef (cf. Figure 2) were applied on a 15% SDS polyacrylamide gel followed by coomassie staining. SDS-PAGE revealed virtually pure Nef. Lanes are labeled with the elution volumes of the analyzed aliquots (57–71). Protein bands of the applied molecular weight marker (M) are labeled with the corresponding molecular weight in kDa.