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. Author manuscript; available in PMC: 2010 Oct 1.
Published in final edited form as: Nat Biotechnol. 2010 Mar 28;28(4):341–347. doi: 10.1038/nbt.1618

Figure 3.

Figure 3

‘Sponge’-mediated silencing of miR-10b in tumor cells is sufficient to inhibit metastasis.

(a) Real-time RT-PCR of miR-10b in 4T1 cells infected with the miR-10b sponge or control sponge. A representative experiment is shown in triplicate along with s.e.m.

(b) Weight of primary mouse mammary tumors formed by 4T1 cells infected with the miR-10b sponge or control sponge.

(c,d) Bright field imaging and H&E staining of the lungs (c) and number of visible lung metastases (d) in mice bearing 4T1 cells infected with the miR-10b sponge or control sponge, at 4 weeks after orthotopic implantation. Arrows indicate lung metastases. Scale bars, 800 µm for bright field imaging; 200 µm for H&E staining. Data in b and d are presented as mean ± s.e.m. (n = 5 mice in each group).