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. Author manuscript; available in PMC: 2011 Apr 6.
Published in final edited form as: Mol Cancer Ther. 2010 Apr 6;9(4):856–868. doi: 10.1158/1535-7163.MCT-09-1113

Figure 3.

Figure 3

Effects of knockdown of DRs on garcinol-induced sensitization of TRAIL. (A) HCT116 cells were transfected with DR5 siRNA, DR4 siRNA, and control siRNA alone or combined. After 48 h, cells were treated with 15 μM garcinol for 24 h, and whole-cell extracts were subjected to Western blotting for DR5 and DR4. (B) Cells were seeded in a chamber slide and transfected with siRNAs. After 48 h, cells were pretreated with 15 μM garcinol for 12 h and then incubated with 25 ng/mL TRAIL for 24 h. Cell death was determined by the Live/Dead Assay. (C) Effects of garcinol on antiapoptotic (Left panel) and apoptotic protein expression (Right panel). HCT116 cells were pretreated with indicated dose of garcinol for 24 h. Whole-cell extracts were prepared and analyzed by Western blotting using the relevant antibodies. For release of cytochrome c and bid proteins cytosolic extracts were prepared as described in ‘Material and Methods’ and analyzed by Western blotting using the relevant antibodies. The same blots were stripped and reprobed with β-actin antibody to verify equal protein loading.