Table III.
Characteristics of transduced normal and dyskeratosis congenita keratinocytes
| Cell Name | Maximum Lifespana | Percent Colony Forming Efficiencyb | Percent Wound Closure (18 Hours)c | Karyotyped |
|---|---|---|---|---|
| N-HSK-1 | 32 pd | 7.0 +/− 0.4 | 87.2 +/− 8.6 | 46, XY[20] (normal) |
| DC-HSK-1 | 18 pd | 2.1 +/− 0.3 | 21.3 +/− 12.7 | 46, XX[20] (normal) |
| TERT-N-HSK-1 | >90 pd | 20.8 +/− 1.6 | 100.0 +/− 0 | 46, XY[20] (normal) |
| TERT-DC-HSK-1 | >90 pd | 11.7 +/− 2.1 | 90.3 +/− 7.9 | 46, XX[20] (normal) |
| E6/E7-N-HSK-1 | >90 pd | 39.3 +/− 4.0 | 79.4 +/− 7.9 | 46, XY, i(8)(q10)[18]/47, XY,+6[2] |
| E6/E7-DC-HSK-1 | >90 pd | 45.2 +/− 6.3 | 44.0 +/− 1.7 | 48, XX, i(8)(q10),+11,+20[20] |
| TERC-DC-HSK-1 | >60 pd | 16.3 +/− 0.3 | 51.7 +/− 1.6 | 46, XX[20] (normal) |
a
Lifespan in cell culture in population doublings (pd). Maximum lifespan shown for untransduced cells. For the transduced cells, a “>” symbol signifies continuous growth.
b
Percent colony forming efficiency on irradiated feeders.
c
Percent scratch wound closure at 18 hours as compared to 0 hours.
d
Cytogenetic analyses were performed within 20 to 30 pd post transduction. The number in bracket represents the number of metaphases out of 20 that exhibited the noted karyotype.