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. 2009 Nov 10;3(2):51–65. doi: 10.1007/s12154-009-0031-9

Fig. 2.

Fig. 2

Cell viability experiments were expressed as a percentage of living CHO-K1 cells. Adherent CHO cells (10,000 cells per well) were incubated for 2 h at 37 °C with various concentrations of CPP-KLAK conjugates, from left to right: 1, 10, 20, and 50 μM of conjugate (compounds 31, Biotin(O2)-(Gly)4-Cys(Acm)-KLAKLAKKLAKLAK-NH2; 32, penetratinS-S-KLAK; 26, (R6/W3)S-S-KLAK; 27, (R6/W2)-[Pra-Gal(OH)]S-S-KLAK; 28, (R6/W2)-[Pra-Gal(CH2)3(OH)]S-S-KLAK; 29, (R6/W2)-[Pra-Gal(CH2)3(OAc)]S-S-KLAK; and 30, (R6/W0)-[Pra-Gal(OH)]3S-S-KLAK). After incubation, the supernatant was removed; a solution of CCK8 cell counting kit was added, and the absorbance was measured at 450 nm after 2 h. Results are expressed as the average of at least three independent experiments performed in triplicate; differences (Student’s test) being * significant, ** very significant, and *** extremely significant from control value (100%). Only the free KLAK peptide and the conjugate with three galactose units 30 (R6/W0)-[Pra-Gal(OH)]3S-S-KLAK did not affect the cell viability, even at 50 μM