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. Author manuscript; available in PMC: 2010 Oct 8.
Published in final edited form as: Oncogene. 2010 Jan 18;29(14):2013–2023. doi: 10.1038/onc.2009.489

Figure 4.

Figure 4

a) Signalling downstream of FGFR2 in amplified cell lines. Indicated cell lines were grown either in 10% serum, or serum starved for 24 hrs, and lysates were made after 1hr exposure to 1μM PD173074 (+), or no exposure (−), as indicated. Lysates were subject to SDS-PAGE and western blotting with antibodies against FGFR2, phosphorylated FRS2-Tyr196, phosphorylated AKT1-Ser473, phosphorylated ERK1/2-Thr202/Tyr204, CCND1, and β-Actin. b) Side-by-side comparison of lysates from MFM223 grown in 10% serum or serum starved, with or without 1μM PD173074, with SKBR3 lysates for comparison.