Figure 4.

Cell morphology changes dependent on HA in collagen I gels. (A) MDA-MB-231 cells (5 × 10⁴) were placed onto collagen matrix either immobilized with HMW HA or without HA in 20% FBS. Fourty-five minutes after placement of cells on matrix, cells were imaged and spreading was quantified. Cells were imaged at 60X magnification while all cells (>150) within a 10X field of view were enumerated. Statistics were calculated using an unpaired student’s t-test. The error bars represent s.d. from three separate experiments. (B) Time lapsed video microscopy was obtained from cells seeded as described in (A) over 1.5 hrs, revealing filopodia formation (1^st panel, white arrowheads) when on collagen and lamellipodia formation (3^rd panel, white arrow) when on collagen + eHA. Scale bar indicates 25 µm.