Table 1.
Object of measurements | Dmin | Dmax | D + SEM |
Control: tubulovesicular extensions connecting neutrophils in the presence of staurosporine, 200 nM | 128 | 188 | 158 ± 10 |
Tubulovesicular extensions connecting S. typhimirium to neutrophils in the presence of staurosporine, 200 nM | 155 | 246 | 197* ± 8 |
Tubulovesicular extensions developed in the presence of Ro 31-8220, 10 µM | 80 | 195 | 135* ± 5 |
Tubulovesicular extensions developed in the presence of diethylamine NONOate, 1 mM | 150 | 240 | 184* ± 10 |
Tubulovesicular extension developed in the presence of cytochalasin D, 5 µg/ml | 115 | 217 | 153 ± 3 |
Human neutrophils were attached to fibronectin-coated substrata during 20 min in the presence of STS, Ro 31-8220 or diethylamine NONOate and for 10 min in the presence of cytochalasin D and fixed for electron microscopy; The cytoneme diameters were measured on scanning electron images; The data presented are the mean values of diameter (D ± SEM) obtained from the measurements of diameters of 15–25 tubular cellular extensions; Data of three independent analogous experiments were summarized; *p < 0.001 as compared to the control value.