Table 1.

Diameter of neutrophil tubulovesicular extensions induced by different agents, as obtained by scanning electron microscopy

Object of measurements	Dmin	Dmax	D + SEM
Control: tubulovesicular extensions connecting neutrophils in the presence of staurosporine, 200 nM	128	188	158 ± 10
Tubulovesicular extensions connecting S. typhimirium to neutrophils in the presence of staurosporine, 200 nM	155	246	197* ± 8
Tubulovesicular extensions developed in the presence of Ro 31-8220, 10 µM	80	195	135* ± 5
Tubulovesicular extensions developed in the presence of diethylamine NONOate, 1 mM	150	240	184* ± 10
Tubulovesicular extension developed in the presence of cytochalasin D, 5 µg/ml	115	217	153 ± 3

Human neutrophils were attached to fibronectin-coated substrata during 20 min in the presence of STS, Ro 31-8220 or diethylamine NONOate and for 10 min in the presence of cytochalasin D and fixed for electron microscopy; The cytoneme diameters were measured on scanning electron images; The data presented are the mean values of diameter (D ± SEM) obtained from the measurements of diameters of 15–25 tubular cellular extensions; Data of three independent analogous experiments were summarized; *p < 0.001 as compared to the control value.