Table 2.

Correlation between mRNA expression levels and immunohistochemistry staining levels as measured by pathologists and automated systems

Stain	Rho (95% CI)	P-value
ER-alpha
Pathologist	0.67 (0.45-0.89)	4×10−12
Aperio	0.73 (0.51-0.95)	4×10−15
TMAx	0.74 (0.52-0.96)	1×10−15
Ariol	0.67 (0.45-0.89)	2×10−12
PR
Pathologist	0.77 (0.55-0.99)	6×10−17
Aperio	0.69 (0.47-0.91)	6×10−13
TMAx	0.73 (0.51-0.95)	1×10−14
Ariol	0.73 (0.51-0.95)	5×10−15
HER-2 †
Pathologist	0.63 (0.41-0.85)	4×10−10
Aperio	0.43 (0.21-0.65)	1×10−4
TMAx	0.49 (0.27-0.71)	4×10−6
Ariol	0.41 (0.19-0.63)	2×10−4
ER-beta
Pathologist	−0.04 (−0.22-0.22)	0.76
Aperio	0.09 (−0.13-0.31)	0.43
TMAx	0.003 (−0.22-0.22)	0.97
Ariol	−0.12 (−0.34-0.1)	0.3
Aromatase †
Pathologist	0.06 (−0.17-0.29)	0.61
Aperio	−0.03 (−0.26-0.2)	0.79
TMAx	0.04 (−0.19-0.27)	0.76
Ariol	0.09 (−0.14-0.32)	0.44

For ER-alpha, PR and ER-beta, the combined (intensity x percent) score was used. For HER2 and aromatase, the intensity score was used.

^†Results are for the expression probe with the highest correlation coefficients.