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. Author manuscript; available in PMC: 2011 Mar 30.
Published in final edited form as: Biochemistry. 2010 Mar 30;49(12):2725–2731. doi: 10.1021/bi100074s

Figure 4.

Figure 4

Fraction of substrate bound as the protonated species (Table 1) versus the substrate pKa (black dots) is fit to Equation 4 (red line) to determine the proton affinity of the active site. Error bars indicate one standard deviation.