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. 2010 Apr;17(4):221–235. doi: 10.1101/lm.1592510

Figure 7.

Figure 7.

Training-induced IEG expression in the LA and MGm/PIN is downstream from ERK/MAPK in the LA and MGm/PIN, respectively. (A) Schematic of behavioral protocol for intra-LA infusion of U0126. Rats were given intra-LA infusion of the vehicle or U0126 (1 µg/side), followed 30 min later by fear conditioning. Rats were sacrificed 2 h following training. (B) Mean (± SEM) percent Arc/Arg3.1, c-Fos, and EGR-1 immunoreactivity from LA punches taken from rats given intra-LA infusions of vehicle (Arc/Arg3.1: n = 7, c-Fos: n = 7, EGR-1: n = 7) or U0126 (Arc/Arg3.1: n = 8, c-Fos: n = 8, EGR-1: n = 8). Here, Arc/Arg3.1, c-Fos, and EGR-1 levels have been normalized to GAPDH levels for each sample. Representative blots for Arc/Arg3.1, c-Fos, EGR-1, and GAPDH can be viewed at the right. (C) Mean (± SEM) percent Arc/Arg3.1, c-Fos, and EGR-1 immunoreactivity from MGm/PIN punches taken from rats in B given intra-LA infusions of vehicle (Arc/Arg3.1: n = 9, c-Fos: n = 9, EGR-1: n = 9) or U0126 (Arc/Arg3.1: n = 8, c-Fos: n = 8, EGR-1: n = 8). Here, Arc/Arg3.1, c-Fos, and EGR-1 levels have been normalized to GAPDH levels for each sample. Representative blots for Arc/Arg3.1, c-Fos, EGR-1, and GAPDH can be viewed at the right. (D) Schematic of behavioral protocol for intra-MGm/PIN infusion of U0126. Rats were given intra-MGm/PIN infusion of vehicle or U0126 (1 µg/side), followed 30 min later by fear conditioning. Rats were sacrificed 2 h following training. (E) Mean (± SEM) percent Arc/Arg3.1, c-Fos, and EGR-1 immunoreactivity from MGm/PIN punches taken from rats given intra-MGm/PIN infusions of vehicle (Arc/Arg3.1: n = 5, c-Fos: n = 5, EGR-1: n = 5) or U0126 (Arc/Arg3.1: n = 7, c-Fos: n = 7, EGR-1: n = 7). Here, Arc/Arg3.1, c-Fos, and EGR-1 levels have been normalized to GAPDH levels for each sample. Representative blots for Arc/Arg3.1, c-Fos, EGR-1, and GAPDH can be viewed at the right. (*) P < 0.05 relative to vehicle-infused rats.