Figure 1.

Comparison of splicing perturbation in the two mouse models. (a) Plot of skip/include ratio array data for an exon from Nfix in wt (filled squares), HSA^LR (open circles) and Mbnl1^ΔE3/ΔE3 (open squares) mice (n = 4). Separation score is the log₂ ratio of skipping probe set intensity versus include probe set intensity in a mutant relative to wild-type (see inset, also see Methods). (b) Diagram showing numbers of aberrant splicing events in HSA^LR mice and Mbnl1^ΔE3/ΔE3 mice with |sepscore| ≥ 0.3. (c) Correlation of sepscore values for aberrant splicing in HSA^LR compared to Mbnl1^ΔE3/ΔE3 mice (Pearson R² = 0.84). (d–f) Validation of members from different classes of events by RT-PCR. (d) Exons either up (Nfix) or down (Tlk) regulated in both HSA^LR and Mbnl1^ΔE3/ΔE3 mice, (e) an exon only affected in Mbnl1^ΔE3/ΔE3 mice, or (f) only affected in HSA^LR mice. The numbers in parentheses represent the length in nt of the affected exon (gray box), shown with flanking exons (white boxes). The RT-PCR products from three individual mice were quantified using the Bioanalyzer, and inclusion rates were calculated. Samples were judged as different from wild type if a t-test indicated that the sample was unlikely to be from the wild type distribution with p < 0.05 (asterisks).