Figure 4.

Test of human DM1 patients for splicing perturbations newly predicted from mouse model microarray data. Skeletal muscle RNA from 3 individual DM1 patients and one normal human control were compared to those from HSA^LR and Mbnl1^ΔE3/ΔE3 mice by RT-PCR. Inclusion rates were determined on a Bioanalyzer. Statistically significant differences from the normal individual human are indicated by an asterisk, however this captures only technical variation in the RT-PCR protocol, as a single original RNA donation was processed in triplicate. For mouse samples, three individuals of each genetic type were compared, so that statistical differences here arise from both biological and technical variation. Note that extent of genetic variation between the humans is unknown (except at the CTG^exp locus), but is likely much greater than the differences between the mouse groups.