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Chemical and genetic knockdown of chondroitin sulfate prevents the proper formation of the AV canal in zebrafish. (A) Wild type Danio rerio heart at 55 hpf. (B) 55 hpf animals treated from 7-48 hpf with DX, an inhibitor of CS formation. (C) 55 hpf animals injected with a morpholino to chondroitin synthase-1 (chys-1). at = atrium; v = ventricle; av = atrioventricular canal forming region.
