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. Author manuscript; available in PMC: 2010 Apr 12.
Published in final edited form as: Dev Dyn. 2009 Dec;238(12):3103–3110. doi: 10.1002/dvdy.22154

Figure 6.

Figure 6

Depletion of chondroitin sulfate results in disruption of multiple signaling pathways. Tbx2b, versican, and notch1b are normally expressed within the atrioventricular canal at 48 hpf (black arrows in (A), (C), and (E)). However, treatment with the compound DX results in the severe downregulation of expression ((B), (D), and (F)). Bmp4 expression remains within the AVC region when treated with DX (black arrow in (G) and (H)), but ventricular expression is upregulated (white arrow in (H)). For those genes that are downregulated, we have included an image of the entire upper body, as all three of these genes stain the head. This is to demonstrate the absence of heart expression is not due to failure of the in situ probe.