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. 2010 Jan 15;61(6):1885–1895. doi: 10.1093/jxb/erp378

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 6. — Molecular analyses of the rice and Arabidopsis transformants. (A) Osta-siR2141 Northern blot and RT-PCR analysis of the OsARF3s. (1, 2) leaves of the CVS transformants and ZH11, respectively; (3, 4) roots of the CVS transformants and ZH11, respectively; ZH11 was at the five-leaf-stage and the PCR was processed for 30 cycles for OsARF3s and 25 cycles for actin. (B) OsARF3s were down-regulated in leaves of the CVS and non-CVS transformants. (1, 2) Leaves of two respective CVS transformants, (3, 4) leaves of two respective non-CVS transformants, (5) leaves of the five-leaf-stage ZH11, (6) leaves of booting stage ZH11. Materials for PCR were sampled three times. PCRs were processed for 30 cycles for OsARF3s and 25 cycles for actin. (C) RT-PCR analysis of HD-ZIPIIIs in SAMs of the CVS transformants: (1) five-leaf-stage ZH11, (2) the CVS transformants. (D) RT-PCR analysis of HD-ZIPIIIs in IMs of the non-CVS transformants. (1) IMs of booting stage ZH11, (2) IMs of the non-CVS transformants; IMs were about 0.5 cm and materials for PCR were sampled three times. PCR was processed for 30 cycles for HD-ZIPIIIs and 25 cycles for actin. (E) Northern blot analysis of miR166 in SAMs/IMs of the transformants and anti-sense miR166a was used as probe. (1) SAMs of the CVS transformants, (2) SAMs of the five-leaf-stage ZH11, (3) IMs of the non-CVS transformants, (4) IMs of booting stage ZH11; IMs in (3) and (4) were about 0.5 cm. (F) In situ hybridization of the OsHB3 gene in SAMs of the CVS transformants. (1) ZH11, anti-sense probe; (2) the CVS transformants, anti-sense probe; (3) ZH11, sense probe; (4) the CVS transformants, sense probe. Bars in 1–4 were 100 μm. (G) Molecular analyses of the Arabidopsis transformants. (1) Seedling of Columbia; (2, 3) seedlings of two respective transformants; antisense Osta-siR2141 was used as the probe in the Northern blot; RT-PCR was processed for 36 cycles for ARF3 and ARF4, and for 24 cycles for actin (Ar). (H) Expression of Wx and OsVP1 genes in seeds of the non-CVS transformants. (1, 2, 3, 4) Seeds of ZH11 at about 1–2, 3–4, 5–6, >10 DAP, respectively; (5, 6, 7, 8) seeds of the non-CVS transformants at about 1–2, 3–4, 5–6, >10 DAP, respectively; Materials for PCRs were sampled three times. PCR was processed for 32 cycles for OsVP1, 29 cycles for Wx, 30 cycles for OsARF3s, and 25 cycles for actin.