Fig. 8.

Transcript levels of carotenoid biosynthesis genes in TAGL1 RNAi lines. Relative transcript levels were determined by quantitative RT-PCR using gene-specific primers on green (grey bars) and red (black bars) fruit. 18S RNA was used as an internal control to normalize the relative level of each transcript. Standard errors of three replicates are shown. IPP, encoding isopentenyl diphosphate; CrtISO, carotenoid isomerase; CYC-B, lycopene β-cyclase (chromoplast specific); and CrtR-b2, β-ring carotene hydroxylase (chromoplast specific).