Table 2.
List of identified proteins from 2D gels of Brachypodium, displayed both on Fig. 2(salt-soluble proteins) and on Fig. 3 (UCT extracted proteins)
| Salt soluble fraction (Fig. 2) | |||||||||
| Spot | Instr | Bradi_1.0 | MW | pI | Nb uni pep | % Cov | Score | e-value | Best homologue protein name |
| 5 | Q-TOF | Bradi1g13040.1 | 64 670 | 7.67 | 5 | 13 | 389 | globulin 3 [Triticum aestivum] | |
| 6 | Q-TOF | Bradi1g13040.1 | 64 670 | 7.67 | 10 | 23 | 634 | globulin 3 [Triticum aestivum] | |
| 7 | Q-TOF | Bradi1g13040.1 | 64 670 | 7.67 | 15 | 33 | 1300 | globulin 3 [Triticum aestivum] | |
| 8 | Q-TOF | Bradi1g13040.1 | 64 670 | 7.67 | 7 | 15 | 516 | globulin 3 [Triticum aestivum] | |
| 9 | Q-TOF | Bradi1g05910.1 | 56 024 | 6.65 | 12 | 27 | 1109 | globulin 2 [Zea mays] | |
| 10 | Q-TOF | Bradi1g05910.1 | 56 024 | 6.65 | 6 | 15 | 422 | globulin 2 [Zea mays] | |
| 11 | Q-TOF | Bradi1g05910.1 | 56 024 | 6.65 | 12 | 23 | 1149 | globulin 2 [Zea mays] | |
| 12 | Q-TOF | Bradi1g05910.1 | 56 024 | 6.65 | 3 | 8 | 171 | globulin 2 [Zea mays] | |
| 15 | Q-TOF | Bradi2g02140.1 | 39 841 | 8.44 | 13 | 40 | 804 | xylanase inhibitor 725OS [Triticum aestivum] | |
| 16 | Q-TOF | Bradi2g02140.1 | 39 841 | 8.44 | 20 | 51 | 1503 | xylanase inhibitor 725OS [Triticum aestivum] | |
| UCT fraction (Fig. 3) | ||||||||||
| Spot | Instr | Bradi_1.0 | Mw | pI | Nb uni pep | % | Score | e value | Best homologue protein name | 11S polypeptide |
| 1 | Q-TOF | Bradi2g38060.1 | 54 748 | 7.63 | 7 | 20 | 524 | 11S globulin [Avena sativa] | non-maturated | |
| 6 | Maldi | Bradi1g13040.1 | 64 670 | 7.67 | 15 | 34 | 236 | 8.10E-20 | globulin 3 [Triticum aestivum] | |
| 7 | Maldi | Bradi1g13040.1 | 64 670 | 7.67 | 41 | 283 | 1.60E-24 | globulin 3 [Triticum aestivum] | ||
| 8 | Q-TOF | Bradi1g13040.1 | 64 670 | 7.67 | 17 | 37 | 1563 | globulin 3 [Triticum aestivum] | ||
| 9 | Maldi | Bradi1g13040.1 | 64 670 | 7.67 | 41 | 253 | 1.60E-21 | globulin 3 [Triticum aestivum] | ||
| 10 | Q-TOF | Bradi1g13040.1 | 64 670 | 7.67 | 21 | 47 | 2017 | globulin 3 [Triticum aestivum] | ||
| 11 | Maldi | Bradi1g13040.1 | 64 670 | 7.67 | 41 | 269 | 4.10E-23 | globulin 3 [Triticum aestivum] | ||
| 12 | Q-TOF | Bradi1g13040.1 | 64 670 | 7.67 | 21 | 48 | 1981 | globulin 3 [Triticum aestivum] | ||
| 14 | Q-TOF | Bradi4g28220.1 | 54 322 | 6.68 | 7 | 15 | 407 | seed storage globulin [Avena sativa ] | ||
| 15 | Q-TOF | Bradi1g05910.1 | 56 024 | 6.56 | 10 | 24 | 928 | globulin 2 [Zea mays] | ||
| 16 | Q-TOF | Bradi1g05910.1 | 56 024 | 6.56 | 10 | 24 | 961 | globulin 2 [Zea mays] | ||
| 17 | Q-TOF | Bradi1g05910.1 | 56 024 | 6.56 | 14 | 36 | 1259 | globulin 2 [Zea mays] | ||
| 18 | Q-TOF | Bradi2g38060.1 | 54 748 | 7.63 | 13 | 35 | 1020 | 11S globulin [Avena sativa] | non maturated | |
| 19 | Maldi | Bradi2g38060.1 | 54 748 | 7.63 | 37 | 162 | 2.00E-12 | 11S globulin [Avena sativa] | non maturated | |
| 20 | Q-TOF | Bradi2g38060.1 | 54 748 | 7.63 | 19 | 52 | 1529 | 11S globulin [Avena sativa] | non maturated | |
| 21 | Maldi | Bradi4g28220.1 | 54 322 | 6.68 | 27 | 128 | 5.10E-09 | seed storage globulin [Avena sativa ] | non maturated | |
| 22 | Maldi | Bradi2g38060.1 | 54748 | 7,63 | 15 | 41 | 164 | 1.30E-12 | 11S globulin [Avena sativa] | non maturated |
| Bradi2g40840.1 | 55314 | 7,68 | 9 | 20 | 72 | 2.2E-3 | 11S globulin [Avena sativa] | |||
| 24 | Q-TOF | Bradi2g38060.1 | 54748 | 7.63 | 16 | 48 | 1255 | 11S globulin [Avena sativa] | non maturated | |
| 31 | Q-TOF | Bradi3g17070.1 | 37141 | 6.77 | 4 | 23 | 260 | prolamin [Brachypodium distachyon] | ||
| 32 | Q-TOF | Bradi3g17070.1 | 37141 | 6.77 | 4 | 19 | 364 | prolamin [Brachypodium distachyon] | ||
| 33 | Q-TOF | Bradi3g17070.1 | 37141 | 6.77 | 5 | 24 | 427 | prolamin [Brachypodium distachyon] | ||
| 34 | Q-TOF | Bradi3g17070.1 | 37141 | 6.77 | 1 | 4 | 82 | prolamin [Brachypodium distachyon] | ||
| 35 | Q-TOF | Bradi3g17070.1 | 37141 | 6.77 | 1 | 4 | 79 | prolamin [Brachypodium distachyon] | ||
| 43 | Q-TOF | Bradi2g38060.1 | 54748 | 7.63 | 15 | 32 | 1322 | 11S globulin [Avena sativa] | acidic polypeptide | |
| 44 | Maldi | Bradi2g38060.1 | 54748 | 7.63 | 25 | 121 | 2.60E-08 | 11S globulin [Avena sativa] | acidic polypeptide | |
| 45 | Q-TOF | Bradi2g38060.1 | 29618 | 5.85 | 5 | 13 | 555 | 11S globulin [Avena sativa] | acidic polypeptide | |
| 46 | Q-TOF | Bradi4g28220.1 | 54322 | 6.68 | 9 | 16 | 671 | seed storage globulin [Avena sativa ] | acidic polypeptide | |
| 47 | Q-TOF | Bradi4g29130.1 | 37213 | 9.15 | 4 | 12 | 397 | hypothetical protein | acidic polypeptide | |
| OsI_06680 [Oryza sativa] | ||||||||||
| On SwissProt Glutelin type-B 5 | ||||||||||
| 48 | Q-TOF | Bradi4g28220.1 | 54322 | 6.68 | 7 | 21 | 525 | seed storage globulin [Avena sativa ] | acidic polypeptide | |
| 49 | Q-TOF | Bradi2g40840.1 | 29817 | 6.58 | 8 | 19 | 535 | 11S globulin [Avena sativa] | acidic polypeptide | |
| 50 | Maldi | Bradi2g38060.1 | 54748 | 7.63 | 25 | 101 | 2.60E-06 | 11S globulin [Avena sativa] | acidic polypeptide | |
| 52 | Q-TOF | Bradi2g38060.1 | 29618 | 5.85 | 4 | 12 | 383 | 11S globulin [Avena sativa] | acidic polypeptide | |
| 55 | Q-TOF | Bradi4g28220.1 | 22137 | 7.2 | 9 | 21 | 818 | seed storage globulin [Avena sativa ] | basic polypeptide | |
| 56 | Q-TOF | Bradi4g28220.1 | 22137 | 7.2 | 8 | 18 | 832 | seed storage globulin [Avena sativa ] | basic polypeptide | |
| 57 | Q-TOF | Bradi2g38060.1 | 29618 | 5.85 | 6 | 13 | 381 | 11S globulin [Avena sativa] | acidic polypeptide | |
| 61 | Q-TOF | Bradi1g50300.1 | 18873 | 7.55 | 4 | 41 | 557 | putative avenin-like a precursor [Triticum aestivum] | ||
| 62 | Q-TOF | Bradi1g50300.1 | 18873 | 7.55 | 4 | 41 | 430 | putative avenin-like a precursor [Triticum aestivum] | ||
| 63 | Q-TOF | Bradi1g50300.1 | 18873 | 7.55 | 4 | 41 | 324 | putative avenin-like a precursor [Triticum aestivum] | ||
The columns correspond to an assigned protein spot number, the mass spectrometry technique used for identification, the protein identity as referred to in the Bradi_1.0 (release May 2009: http://www.brachypodium.org), its theoretical molecular weight (Mw), its isoelectric point (pI), the number of unique peptides matched, the per cent sequence coverage, the MASCOT individual ion score (LC-MS/MS on the QTOF instrument) or expected value for the protein hit (MALDI-MS), the NCBI database best homologue protein name and the type of 11S polypeptide obtained from mass spectrometry analysis of the peptides.