Table 3.
List of the in silico predicted storage proteins found among Bradi_1.0 sequences
| Brachypodium sequences | e-value | Homologue | Protein name | Exp | % Exp |
| (UniRef100) | |||||
| Type 11S | |||||
| Bradi2g38050.1 | 0 | Q38780 | 11S globulin OS=Avena sativa | yes | 40–50 |
| Bradi2g38060.1 | 0 | Q38780 | 11S globulin OS=Avena sativa | yes | |
| Bradi2g40840.1 | 0 | Q38780 | 11S globulin OS=Avena sativa | yes | |
| Bradi4g28220.1 | 0 | P12615 | 12S seed storage globulin 1 OS=Avena sativa | yes | |
| Bradi4g29130.1 | 3E-080 | O49258 | 12S globulin OS=Avena sativa | yes | |
| Bradi2g20860.1 | 5E-034 | Q0Q5E3 | Globulin 1 Tax=Triticum aestivum | ||
| Bradi2g37470.1 | 1E-178 | B6SLE7 | Legumin-like protein OS=Zea mays | ||
| Bradi2g37470.2 | 4E-141 | B6SLE7 | Legumin-like protein OS=Zea mays | ||
| Bradi2g37860.1 | 2E-158 | O49258 | 12Sglobulin OS=Avena sativa | ||
| Bradi2g38070.1 | 4E-147 | Q38780 | 11S globulin OS=Avena sativa | ||
| Bradi2g62590.1 | 8E-142 | B6TDD3 | Legumin-like protein OS=Zea mays | ||
| Bradi2g62590.2 | 6E-171 | B6TDD3 | Legumin-like protein OS=Zea mays | ||
| Type 7S | |||||
| Bradi1g05910.1 | E-138 | Q7M1Z8 | Globulin-2 OS=Zea mays | yes | 9–10 |
| Bradi1g13040.1 | 2E-159 | B7U6L4 | Globulin 3 OS=Triticum aestivum | yes | |
| Bradi1g63180.1 | 2E-164 | B6SK46 | Cupin family protein OS=Zea mays | ||
| Prolamin | |||||
| Type S-rich | |||||
| Bradi1g50300.1 | 2E-024 | Q2A784 | Putative avenin-like a OS=Triticum aestivum | yes | 3–4 |
| Bradi3g17070.1 | 2E-155 | C3SAE7 | Prolamine OS=Brachypodium distachyon | yes | |
| Bradi1g50200.1 | 3E-024 | Q2A784 | Putative avenin-like a OS=Triticum aestivum | ||
| Bradi1g50290.1 | 7E-024 | Q2A781 | Putative avenin-like a OS=Aegilops tauschii | ||
| Bradi2g33280.1 | 3E-025 | C3SAE7 | Prolamine OS=Brachypodium distachyon | ||
| Bradi2g38530.1 | 3E-11 | C3SAE7 | Prolamine OS=Brachypodium distachyon | ||
| Bradi2g39940.1 | 2E-028 | C3SAE7 | Prolamine OS=Brachypodium distachyon | ||
| HMW | |||||
| Bradi2g20870.1 | 7E-14 | Q8LKI8 | HMW glutenin subunit y | ||
| OS=Aegilops speltoides | |||||
| Bradi2g20910.1 | 2E-015 | Q670Q5 | High molecular weight glutenin subunit | ||
| 1Dy10.1 OS=Triticum aestivum |
The columns correspond to putative Brachypodium storage protein sequences (Bradi_1.0), e-value, entry number of the best homologue sequence as found in the Uniref100 databank and corresponding protein name, evidence at proteomic level (‘yes’ means that the gene was found to be expressed based on our 2D-E experiments), and approximate percentage of the total extractable proteins and estimated from 2D-E analysis. Three protein families are indicated: 11S globulin, 7S globulins, prolamins (divided into S-rich prolamins and HMW prolamins).