Skip to main content
. 2010 Mar 26;61(6):1853–1867. doi: 10.1093/jxb/erq056

Fig. 3.

Fig. 3.

Embryo germination pattern in tobacco starts from the culture of 5 DAP ovules. (A) A seedling with two symmetrical cotyledons and normal leaf primordia. (B–G) Abnormal seedlings germinated from the ovules treated with TIBA. (B) An abnormal seedling without cotyledons. (C–G) Abnormal seedlings with a cup-shaped cotyledon (C), single cotyledon (D), two asymmetric cotyledons (E, F), and multiple cotyledons (G). (H) A seedling with a cup-shaped cotyledon and normal leaves. The seedling was not transplanted to the normal medium until the embryo germinated in TIBA-treated medium. Arrowheads indicate leaf primordia or leaves in (A, F, H). (I) Effects of IAA and TIBA on the germination frequency of cultured embryos, showing average and the standard error (s.e.) * 0.01 <P <0.05 indicates a significant difference; ** P <0.01 indicates extremely significant difference. Bar=2 mm. (This figure is available in colour at JXB online.)