Fig. 5.

Western blot of ABP1 and PM H⁺-ATPase in tobacco ovules. (A) ABP1 recognized by polyclonal anti-maize ABP1 antibody. (B) PM H⁺-ATPase recognized by polyclonal antibody which comes from Nicotiana plumbaginifolia. In order to exclude the possible interference of BSA, which is part of the antibody dilution buffer and could make a molecular weight marker, the whole NC membrane (transferred with the SDS-PAGE) was hybridized with the antibody of PM H⁺-ATPase. TP, total protein; BSA, bovine serum albumin; ATPase, plasma membrane H⁺-ATPase.