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. 2010 Mar 26;61(6):1853–1867. doi: 10.1093/jxb/erq056

Fig. 8.

Fig. 8.

Immunolocalization of ABP1 and PM H+-ATPase in tobacco abnormal embryos treated with TIBA in vitro. (A–J) Bright-field images. (A, B, F, G) Abnormal early globular embryos from the culture of 3 DAP ovules have lost the apical–basal polarity (A, F) and asymmetrical division in the embryo proper (B, G). (a1, b1, f1, g1) The corresponding DAPI nuclear counterstain. (a2, b2, c–e) The corresponding ABP1 immunofluorescence images in (A–E). (f2, g2, h–j) The corresponding PM H+-ATPase immunofluorescence images in (F–J). (C, H) Abnormal undifferentiated embryos from the culture of 5 DAP ovules. (D, E, I, J) Abnormal differentiated embryos with cup-shaped cotyledon primordia, single cotyledon primordia, three cotyledon primordia, and asymmetric cotyledons from the culture of 5 DAP ovules, respectively. (A, B, F, G) Bar=20 μm; (C–E, H–J) bar=40 μm. (This figure is available in colour at JXB online.)