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. 2010 Mar 26;61(6):1853–1867. doi: 10.1093/jxb/erq056

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© 2010 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 9. — Immunogold localization of IAA, ABP1, and PM H⁺-ATPase in the early globular embryo (5 DAP) of tobacco. (A) Semi-thin section of an early globular embryo. (B–H) Ultrathin sections of the early globular embryo, showing IAA, ABP1, and PM H⁺-ATPase immunogold granules, respectively. (B) Numerous IAA gold particles appeared in the cytoplasm near the cell wall, and a few were distributed in the plasma membrane (PM) and cell wall of the embryo. (C) Many IAA gold particles accumulated in the nucleus. (D) A control section without the primary IAA antibody, and the secondary antibody was a goat anti-rat IgG antibody. The image showed no gold particles. (E, F) ABP1 gold particles mainly distributed in the PM and cytoplasm near the cell wall (E) and in the ER (F). (G) Nearly all PM H⁺-ATPase gold particles appeared in the PM. Arrows indicate the gold particles. (H) A control section without the primary antibodies of ABP1 or PM H⁺-ATPase, while the secondary antibody was goat anti-rabbit IgG antibody. The image showed no gold particles. (A) Bar=25 μm; (B) bar=5 μm; (C–E) bar=0.2 μm; Cy, cytoplasm; CW, cell wall; ER, endoplasmic reticulum; N, nucleus; Nu, nucleolus; Pl, plastid; PM, plasma membrane.