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. 2010 Feb 23;20(4):279–289. doi: 10.1016/j.cub.2009.12.059

Figure 1.

Figure 1

Model of the Cohesin Hinge from Yeast

(A) Model of the yeast cohesin hinge based on the bacterial Smc hinge from Thermotoga maritima. Amino acid residues used for the mutation at the dimerization surfaces of the Smc1 and Smc3 hinge domains are indicated.

(B) Sequence alignment of the hinge domain showing the conservation of F584 and M665 in Smc1 and M577 in Smc3.

(C) Mutations and their phenotype. The rightmost column represents the ability of ectopic copies of wild-type SMC1/SMC3 or mutant smc1/smc3, integrated into strains heterozygous for Δsmc1/Δsmc3, to rescue the deletion in tetrad dissection assays. Ideally, one rescue per tetrad is expected if the ectopic gene can complement the deletion of the endogenous gene. (See also Figure S1.)