Table 2.
Changes in equilibrium binding free energy of STX and TTX for the outer vestibule mutants (From Penzotti et al. [51]).
| Mutation | ΔΔGSTX ± SE | ΔΔGTTX ± SE | ΔΔGSTX/ΔΔGTTX ± SE |
|---|---|---|---|
| D400A | 3.8 ± 0.1 | 3.3 ± 0.3 | 1.2 ± 0.1 |
| Y401D | 2.4 ± 0.1 | 5.2 ± 0.3 | 0.5 ± 0.2 |
| Y401C | 2.7 ± 0.1 | 4.8 ± 0.3 | 0.6 ± 0.1 |
| E403Q | 6.2 ± 0.1 | 5.2 ± 0.3 | 1.2 ± 0.05 |
| E755A | 5.7 ± 0.1 | 5.4 ± 0.3 | 1.1 ± 0.1 |
| E758Q | 6.0 ± 0.1 | 3.3 ± 0.3 | 1.8 ± 0.05 |
| K1237A | 3.9 ± 0.2 | 4.1 ± 0.3 | 1.0 ± 0.1 |
| M1240E | 3.9 ± 0.1 | 4.8 ± 0.3 | 0.8 ± 0.1 |
| M1240K | 6.1 ± 0.2 | 5.4 ± 0.3 | 1.1 ± 0.1 |
| D1532N | 6.2 ± 0.2 | 2.4 ± 0.3 | 2.6 ± 0.1 |