. Author manuscript; available in PMC: 2011 Jan 1.

Published in final edited form as: J Anal At Spectrom. 2010;25(3):260–268. doi: 10.1039/b921770c

Table 3.

Averages of the integrated ion intensities over the transient signals for individual KG1a, U937 cells, and AA120-Eu beads.

Samples		Free Cells		Cells and beads			Free beads
Samples		¹⁶⁹Tm	¹⁹³Ir	¹⁶⁹Tm	¹⁹³Ir	¹⁵³Eu	¹⁵³Eu
KG1a stained with ¹⁶⁹Ir interchelator and CD34-¹⁶⁹Tm	K1	33000	49000	33000	46000	8000	7000
	K2	62000	44000	63000	43000	7000	7000
	K3	71000	44000	72000	49000	7000	7000
	K4	17000	48000	17000	47000	8000	7000
	K5	42000	48000	41000	48000	7000	7000
	K6	59000	48000	60000	50000	7000	7000
	K8	36000	49000	36000	48000	8000	7000
	K9	44000	47000	39000	52000	8000	7000
	K10	16000	51000	16000	50000	8000	7000
	K11	28000	51000	27000	50000	8000	7000
	K12	30000	48000	30000	52000	8000	8000
U937 stained with only ¹⁶⁹Ir interchelator	U1	160	51000	166	51000	10000	9000
	U2	190	51000	201	49000	9000	9000
	U3	280	50000	278	53000	9000	9000
	U4	460	52000	454	51000	9000	9000
	U5	3300	51000	3697	56000	9000	9000
	U6	1900	55000	434	50000	9000	9000
	U7	530	50000	772	50000	9000	9000
	U8	590	50000	531	50000	10000	9000
	U9	1700	50000	1787	50000	10000	9000
	U10	390	51000	411	54000	9000	9000
	U11	280	53000	324	53000	9000	8000
	U12	460	52000	771	46000	9000	8000

12 different clones of CD34 antibodies:

K (KG1a cells stained with ¹⁶⁹Ir intercalator and Tm-labeled CD34) and U (U937 cells stained only with ¹⁶⁹Ir intercalator). Each sample was examined in a 100:1 mixture with metal-containing beads AA120-Eu. The Eu content of the AA120-Eu gated without cells is reported on the last column.