Kinetic measurements for the binding of monomer protein to DNA E-box. Data shows the time dependent increase in anisotropy after rapid mixing (A) 50 nM FITCMax (final concentration) with 1 µM DNA E-box (final concentration), (B) Max-DNA complex with 1 µM Max, (C) Max-DNA complex with 1 µM Myc, and (D) Max-DNA complex with 1 µM Mad, respectively, at 21°C.