Table 5.
Number ofAAV-TF-RhEpo-2.3w vector genome copies found in various tissues at necropsy after gene transfer to the right parotid gland of rhesus macaque #6851*
| Tissue (n) | vg copies/300ng DNA |
|---|---|
| Right parotid (3) | 4.1×103 |
| Left Parotid. (2) | 60 |
| Right submandibular lymph node (3) | 300 |
| Left submandibular lymph node (2) | 34 |
| Serum (2) | 21 |
| Liver (3) | 2.8×103 |
| Right masseter (2) | 60 |
| Left masseter (2) | 60 |
| Lung (3) | 32 |
| Tracheobronchial lymph node (3) | 129 |
*
The data shown are the mean number of vector genome (vg) copies/300ng DNA determined with genomic DNA extracted from the indicated tissues 76 days after administration of 1.5×1012 vg to the right parotid gland of macaque #6851. This animal was euthanized and necropsied as described in the text. (n) = the number of separate tissue pieces from which genomic DNA was extracted. The results shown are representative of those with each respective tissue piece examined. DNA from each extracted tissue sample was then assayed in triplicate by QPCR. See text for additional details.