FIGURE 4.

The requirement of BAK/BAX for ceramide generation during apoptosis is not specific to the apoptotic stimulus or cell type. a, viability of hematopoietic cells following the removal of IL-3. b, ceramide was measured in the cells in a 8 h following removal of IL-3. c, viability of hematopoietic cells treated with 10 μm cisplatin. d, ceramides were measured in the cells in c 8 h after cisplatin treatment. The average of three independent experiments is shown. Error bars, ±S.D. For statistical analyses a two-way ANOVA was performed with a Bonferroni post-test. *, p ≤ 0.05 statistically significant difference between treatment and its corresponding untreated control and #, p ≤ 0.05 statistically significant difference between DKO and the corresponding time-matched WT value.