FIGURE 5.
Ceramide generation during apoptosis is independent of MOMP. a and b, WT BMK cells were treated with 30 μm R-t-Bid-BH3 for 6 h. Viability (a) was measured by trypan blue exclusion and (b) long-chain ceramides were measured. c, WT and BCL-XL-overexpressing (BCL-XL OE) hematopoietic cells were treated with cisplatin (10 μm) and (a) viability was measured at the indicated times via vital dye exclusion (4′,6-diamidino-2-phenylindole) and flow cytometry and (b) long-chain ceramides measured at 8 h. a, c, and d, data represent the average of three independent experiments. Error bars, ±S.D. b, data are the mean of duplicates from each of two independent experiments. Error bars, ±S.E. For statistical analyses a two-way ANOVA was performed with a Bonferroni post-test. *, p ≤ 0.05 statistically significant difference between treatment and its corresponding untreated control and #, p ≤ 0.05 statistically significant difference between DKO and the corresponding time-matched WT value. ns, no significant difference.