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. 2010 Feb 17;285(16):11846–11853. doi: 10.1074/jbc.M109.066399

FIGURE 3.

FIGURE 3.

Deletion and mutation of the miR-27b binding site reverses PPARγ1 mRNA decay. A, sequence of the AU-rich PPARγ-3′-UTR. The miR-27 binding site is underlined, whereas ARE 1 sites (AUUUA) are shaded, and ARE 4 (12-mer A/U with maximum one mismatch) sites are marked with boxes. B, alignment of the PPARγ-3′-UTR with the miR-27b sequence and the sequences of the construct pGL3-PPARγ-3′UTR-ΔmiR-27 and pGL3-PPARγ-3′UTR-C83A/U84G. Mutated nucleotides are underlined. C, differentiated THP-1 cells were transfected with pGL3-control, pGL3-PPARγ-3′UTR, pGL3-PPARγ-3′UTR-ΔmiR-27, or pGL3-PPARγ-3′UTR-C83A/U84G and luciferase expression was measured after stimulation with 1 μg/ml LPS for 3 h. Basal activity was set to 1, ratios of 3′-UTR constructs/pGL3-control are displayed. Data present mean values ± S.E., n ≥ 3. Statistics were analyzed with the unpaired Student's t test. *, p < 0.05; **, p < 0.01.