FIGURE 4.

NFκB-dependent miR-27b expression. A, miR-27b expression was measured in primary human macrophages in response to LPS (1 μg/ml, 2 h) by qPCR. To investigate a role of NFκB, cells were prestimulated for 1 h with 10 μm Bay11–7082. Basal expression was set to 1. B, primary human macrophages were pretreated with Bay as in A followed by 3 h of LPS exposure. PPARγ1 mRNA was determined by qPCR. C, temporal pattern of PPARγ1 mRNA and miR-27b expression was measured by qPCR after stimulation with 1 μg/ml LPS. Data represent mean values ± S.E., n ≥ 4. Statistics were analyzed with the paired Student's t test. *, p < 0.05.