FIGURE 5.

Inhibition of miR-27b reverses PPARγ1 mRNA destabilization. A, primary human macrophages were transfected with different concentrations (50, 100, 150 pmol) of anti-miR-27b or a negative control. After transfection, cells were stimulated with 1 μg/ml LPS for 3 h, and PPARγ1 mRNA level was determined by qPCR. B, primary human macrophages were transfected with 150 pmol of anti-miR-27b or negative control and PPARγ1 mRNA half-life was determined by stimulating cells with 100 μm DRB and 1 μg/ml LPS for 1 to 3 h. Data represent mean values ± S.E., n ≥ 4. Statistics were analyzed with the paired Student's t test. **, p < 0.01; ***, p < 0.001.