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Effect of CoA on NAD-ME2 (A) and NAD-MEH (B) activity as a function of the malate concentration is shown. Assays were done at 4 mm NAD and 10 mm MnCl2 in the presence of the indicated CoA concentration. The Km for l-malate of NAD-ME2 (C) and S0.5 for malate of NAD-ME-H (D) as a function of CoA concentration is indicated as an inset in each graph. The estimated kcat and Km or S0.5 for l-malate of each enzyme in the presence of 50 μm CoA are indicated in Table 3.
