TABLE 1.

Kinetic properties of recombinant Arabidopsis NAD-ME1, -2, and -H

The indicated values are the average of at least three different experiment with no more than 5% S.D. among them.

	pH optimum	kcat	Km NAD	kcat/Km NAD	Kml-malate	kcat/Kml-malate
		s−1	mm		mm
NAD-ME1a	6.4	31.1	0.50	60.2	3.0b (1.9)	10.3
NAD-ME2a	6.6	44.1	0.50	88.2	3.0	14.7
NAD-MEH	6.5	39.0	0.55	67.0	2.7b (2.0)	14.2
NAD-ME1q	6.6	32.1	0.52	61.7	0.2	160.5
NAD-ME2q	6.4	11.4	0.58	19.6	4.0a (2.1)	2.9

^a Values previously obtained (12) are included for comparison.

^b S_0.5; the calculated Hill coefficient (n_H) is indicated in parentheses.